Sub-titles are only used for uniqueness purposes and cannot be spotted or otherwise used...the example usually used is
Ulaire Attea, The Easterling. He's not an
Easterling, you can't use him as a requirement for spotting an
Easterling, etc.
Now, if you were to create a card that said something like "Bearer must be
Galadriel, Lady of Light" that would be permissible, if incredibly narrow. I suppose you could even stretch that to "Bearer must have sub-title of
Lady of Light", but that's kind of awkward.
Like I said, there is no use within the game for subtitles, outside of reference purposes for players to differentiate different versions of unique cards.